Click on the table header to choose the columns to plot and then right click. Chose the 'Plot' option from the pop-up menu and then the type of plot you want.
You can also use the plot assistant: press 'CTRL+ALT+W' keys to show it, or go to 'Plot' menu -> 'Plot wizard'.
Go to the 'Data' menu->'Select data range'. Click in the plot window and use the 'Up' and 'Down' arrows keys to select the curve to analyse. Keeping 'CTRL' button and 'Left' or 'Right' arrow keys simultaneousely pressed permit to move the selected cursor and consequently to modify the data range.
Go to the 'Analysis' menu->'Fit Wizard...'. Define the function (myFunction=...), enter the initial guesses for the parameters, choose the fitting range and the number of iterations and click 'OK'.
Right click on the image you want to analyse and select the option View pixel line profile from the popup menu. A dialog window opens and allows you to select the number of pixels used for the analysis. Choose a value and press the OK button. Then click on the image to select the start point and move your mouse to select an end point while keeping the left button pressed. When you release the left button a plot window appears, representing the pixel intensity versus pixel index.
Open the Plot details dialog, select the layer you want to edit,
uncheck the option Synchronize scales and press the Apply button. This way the opposite scales become independent.
In order to make this behaviour the default for all new 2D plot layers, open the Axes tab of the 2D Plots preferences dialog,
uncheck the option Synchronize scale divisions and press the Apply button.