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Figure 4. The protonophore CCCP inhibits Icm/Dot-dependent activities.

A. Effect of translation inhibitors (kanamycin, erythromycin) and protonophore (CCCP) on translocation of the LepA effector in J774 cells. B. Icm/Dot-dependent red blood cell (RBC) lysis by L. pneumophila and effect of translation inhibitors (kanamycin, erythromycin) and respiratory chain poison sodium azide. C. Time course effect of the CCCP protonophore or the DMSO carrier on Icm/Dot-dependent RBC lysis by L. pneumophila. D. Effect of the CCCP protonophore on the bacterial ATP levels as a function of exposure time. In all panels error bars represent standard deviation from three independent experiments.

This figure was published in "Chemical Genetics Reveals Bacterial and Host Cell Functions Critical for Type IV Effector Translocation by Legionella pneumophila", Xavier Charpentier, JoŽlle E. Gabay, Moraima Reyes, Jing W. Zhu, Arthur Weiss, Howard A. Shuman, PLoS Pathogens.